Antibody Conjugation Services
Antibody Conjugation – reproducibility and consistency
High-quality, reproducible antibody conjugation is essential for consistent experimental results. Our proprietary purification processes have been developed to maximize the yield, purity, and immunoreactivity of your antibodies.
- Conjugation of small quantities through to industrial scale large batches.
- Extensive range of markers for conjugation: including alkaline phosphatase, biotin, HRP and the fluorescent dyes summarised below.
| Dye | Laser excitation line (nm) |
Maximal absorbance (nm) | Maximal emission (nm) | Suggested laser | Fluores- cence color |
|---|---|---|---|---|---|
| Alexa Fluor® 405*(1) | 405, 407 | 401 | 421 | Violet diode | |
| Pacific Blue™*†(1) | 405, 407 | 416 | 456 | Violet diode | |
| FITC | 488 | 495 | 525 | Argon-Ion | |
| Alexa Fluor® 488(1) | 488 | 495 | 519 | Argon-Ion | |
| RPE | 488 | 490, 565 | 578 | Argon-Ion | |
| APC | 633, 635, 647 | 650 | 661 | Krypton-Argon | |
| Alexa Fluor® 647(1) | 633, 635, 647 | 650 | 665 | Krypton-Argon | |
| PE-Alexa Fluor® 647#(1) | 488 | 496, 546 | 667 | Argon-Ion | |
| PE-Alexa Fluor® 750(1) | 488 | 496, 546 | 779 | Argon-Ion | |
| DyLight™ 488 | 493 | 488 | 518 | Argon-Ion | |
| DyLight™ 549 | 550 | 549 | 568 | Krypton-Argon | |
| DyLight™ 649 | 646 | 649 | 674 | Krypton-Argon | |
| Alexa Fluor® 700(1) | 675-700 | 696 | 719 | Xenon-arc |
(1) For conjugation to AbD Serotec primary antibodies only.
* Can only be used on flow cytometers with a violet laser (402–415 nm range).
† Low spectral overlap with green fluorochromes e.g. FITC.
# Caution: if you intend to use PE-Alexa Fluor® 647 in conjunction with APC or Alexa Fluor® 647, please be aware of the possibility of excitation in the tandem dye directly by the 633/635/647 laser.
Alexa Fluor® and Pacific Blue™ are trademarks of Molecular Probes Inc, OR, USA.
DyLight™ is a trademark of Thermo Fisher Scientific and its subsidiaries.
Purification
Our proprietary purification processes are designed to maximize the yield, purity, and immunoreactivity of your antibodies. We offer protein G, protein A and ion exchange chromatography purification processes. The method selected is based on the antibody provided and/or customer requirements.
Quality Control
We test and validate all antibodies according to our ISO 9001-accredited design and production quality assurance system. Antibody purity is assessed by SDS-PAGE, HPLC, or IEF. Our comprehensive testing facility includes ELISA, DyLight.
Confidentiality
We treat all data and material that you provide us as completely confidential, right down to your name and affiliation. A signed Confidential Disclosure Agreement is available on request.
Customer Satisfaction
We pride ourselves on our flexibility towards customers’ needs and the highest quality pre- and post-production technical support. Our friendly and knowledgeable staff aim to answer all enquiries within a day.
